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KMID : 1007519970060030229
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Food Science and Biotechnology
1997 Volume.6 No. 3 p.229 ~ p.230
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Increased Resistance to Oxidative Stress by Cloned Mn-SOD Gene in Escherichia coli
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So Jae-Seong
Chang Woo-Suk
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Abstract
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The manganese superoxide dismutase(Mn-SOD) is a metalloenzyme which plays an important role in protecting cells against oxygen toxicity. The cloned Mn-SOD gene of Escherichia coli was subcloned into the E. coli-Lactococcus spp. shuttle vector pGKV21 to make the plasmid pGKV-SOD. The pGKV-SOD was then transformed into the E. coli strain HB101 by electroporation. SOD activity of the resulting transformant was 26.5% higher than that of E. coli harboring pGKV21 vector itself. In line with this result, the E. coli transformant showed the increased resistance to paraquat-mediated inhibition of growth. Paraquat is known as a promoter to the formation of a superoxide radical anion, O^￣_2Based on these data, we can conclude that the cloned Mn-SOD provides protection in E. coli against oxygen toxicity.
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